The “tune” you uploaded to ecu blocked obd access.
That things happen when you flash to ecu files that are “from not known and trustworthy father”.
Next thing to try to gain access again is dismantle ecu and do BDM.
But following your career with this car in forum i encourage you to stop playing with it yourself and go to a local experienced tuner, pay for a good work and be happy enjoying your well remapped car. Otherwise i will see you in forum in next days complaining about a good car converted by yourself games in a non starter. Just a friendly advice.
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